By means of a questionnaire survey of 1104 college students,the paper studies the present situation of college students’ self - differentiation,self - hindrance and learning burnout and the relationship between them. The results indicate that the college students’self - differentiation is in the middle level,and there are significant differences in sex and poverty,and the degree of self - hindrance and learning burnout is lower,and the difference is significant in whether they are poor or not. Self - differentiation,self - hindrance and learning burnout have significant correlations with each other. What’s more,self - differentiation and self - hindrance have a significant predictive effect on learning burnout,and the mediating effect between self - differentiation and learning burnout is significant and plays a mediating role.